Characterisation of guinea pig precision-cut lung slices: comparison with human tissues
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چکیده
منابع مشابه
Characterisation of guinea pig precision-cut lung slices: comparison with human tissues.
Precision-cut lung slices (PCLS) allow comparison of the airway responses of different species under identical experimental conditions. The aim of this study was to establish and characterise PCLS from guinea pigs (GPs) and to compare them with human PCLS. GP PCLS were prepared according to previously published procedures with the exception that the agarose solution and the initial incubation m...
متن کاملCharacterization of guinea pig precision-cut lung slices: Comparison with human tissues
Precision-cut lung slices (PCLS) allow comparison of airway responses of different species under identical experimental conditions. Our aim was to establish and characterize PCLS from guinea pigs (GP) and to compare them to human PCLS. Guinea pig PCLS were prepared according to previously published procedures with the exception that the agarose solution and the initial incubation medium contain...
متن کاملBiaxial distension of precision-cut lung slices.
The mechanical forces acting on lung parenchyma during (mechanical) ventilation and its (patho)physiological consequences are currently under intense scrutiny. Several in vivo and cell culture models have been developed to study the pulmonary responses to mechanical stretch. While providing extremely useful information, these models do also suffer from limitations in being either too complex fo...
متن کاملElectric field stimulation of precision-cut lung slices.
The precision-cut lung slice (PCLS) technique is widely used to examine airway responses in different species. We developed a method to study nerve-dependent bronchoconstriction by the application of electric field stimulation (EFS) to PCLS. PCLS prepared from Wistar rats were placed between two platinum electrodes to apply serial rectangular impulses (5-100 Hz), and bronchoconstriction was stu...
متن کاملReproducible uniform equibiaxial stretch of precision-cut lung slices.
Simulating ventilator-induced lung injury (VILI) in the laboratory requires stretching of lung alveolar tissue. Whereas precision-cut lung slices (PCLSs) are widely used for studying paracrine signaling pathways in the lungs, their use in stretch studies is very limited because of the technical challenge of fixing them to a stretchable substrate, stretching them uniformly, or holding them in a ...
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ژورنال
عنوان ژورنال: European Respiratory Journal
سال: 2006
ISSN: 0903-1936,1399-3003
DOI: 10.1183/09031936.06.00004206